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Upstream promoter sequences and αCTD mediate stable DNA wrapping within the RNA polymerase–promoter open complex
Author(s) -
Cellai Sara,
Mangiarotti Laura,
Vannini Nicola,
Naryshkin Nikolai,
Kortkhonjia Ekaterine,
Ebright Richard H,
Rivetti Claudio
Publication year - 2007
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400888
Subject(s) - rna polymerase , promoter , biology , dna , microbiology and biotechnology , polymerase , protein subunit , transcription (linguistics) , escherichia coli , gene , genetics , gene expression , linguistics , philosophy
We show that the extent of stable DNA wrapping by Escherichia coli RNA polymerase (RNAP) in the RNAP–promoter open complex depends on the sequence of the promoter and, in particular, on the sequence of the upstream region of the promoter. We further show that the extent of stable DNA wrapping depends on the presence of the RNAP α‐subunit carboxy‐terminal domain and on the presence and length of the RNAP α‐subunit interdomain linker. Our results indicate that the extensive stable DNA wrapping observed previously in the RNAP–promoter open complex at the λ P R promoter is not a general feature of RNAP–promoter open complexes.