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Mammalian SGO2 appears at the inner centromere domain and redistributes depending on tension across centromeres during meiosis II and mitosis
Author(s) -
Gómez Rocío,
Valdeolmillos Ana,
Parra María Teresa,
Viera Alberto,
Carreiro Candelas,
Roncal Fernando,
Rufas Julio S,
Barbero José L,
Suja José A
Publication year - 2007
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400877
Subject(s) - centromere , cohesin , prometaphase , biology , kinetochore , meiosis , meiosis ii , microbiology and biotechnology , telophase , establishment of sister chromatid cohesion , mitosis , prophase , chromosome segregation , sister chromatids , genetics , metaphase , chromosome , gene
Shugoshin (SGO) is a family of proteins that protect centromeric cohesin complexes from release during mitotic prophase and from degradation during meiosis I. Two mammalian SGO paralogues—SGO1 and SGO2—have been identified, but their distribution and function during mammalian meiosis have not been reported. Here, we analysed the expression of SGO2 during male mouse meiosis and mitosis. During meiosis I, SGO2 accumulates at centromeres during diplotene, and colocalizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres. However, SGO2 and RAD21 change their relative distributions during telophase I when sister‐kinetochore association is lost. During meiosis II, SGO2 shows a striking tension‐dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis. We propose a model by which the redistribution of SGO2 would unmask cohesive centromere proteins, which would be then released or cleaved by separase, to trigger chromatid segregation to opposite poles.