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The CRY box: a second APC cdh1 ‐dependent degron in mammalian cdc20
Author(s) -
Reis Alexandra,
Levasseur Mark,
Chang HengYu,
Elliott David J,
Jones Keith T
Publication year - 2006
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400772
Subject(s) - cdc20 , degron , microbiology and biotechnology , mitosis , biology , metaphase , anaphase , anaphase promoting complex , cdh1 , ubiquitin ligase , chemistry , ubiquitin , genetics , cell cycle , cancer , gene , cadherin , cell , chromosome
Cdc20 and cdh1 are coactivators of the anaphase‐promoting complex (APC). APC cdc20 is necessary for the metaphase–anaphase transition and, at the end of mitosis, vertebrate cdc20 itself becomes a target for degradation through KEN‐box‐dependent APC cdh1 activity. By studying the degradation of fluorescent protein chimaeras in mammalian oocytes and early embryos, we found that cdc20 was degraded through two independent degradation signals (degrons), the KEN box and a newly described CRY box. In both oocytes and G1‐stage embryos, the rate of degradation through the CRY box was greater than through the KEN box, although both were mediated by APC cdh1 . Thus, mammalian oocytes and embryos have the capacity to recognize two degrons in cdc20.