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The replication kinase Cdc7‐Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating cell nuclear antigen
Author(s) -
Gérard Annabelle,
Koundrioukoff Stéphane,
Ramillon Vincent,
Sergère JeanChristophe,
Mailand Niels,
Quivy JeanPierre,
Almouzni Geneviève
Publication year - 2006
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400750
Subject(s) - origin recognition complex , proliferating cell nuclear antigen , replication factor c , licensing factor , microbiology and biotechnology , eukaryotic dna replication , dna replication factor cdt1 , biology , dna replication , chromatin , control of chromosome duplication , histone , protein subunit , nucleosome , pre replication complex , s phase , dna , genetics , gene
The coordination of chromatin assembly with DNA replication, which is essential for genomic stability, requires the combined activation of histone deposition with the firing of replication origins. We report here the direct interaction of chromatin assembly factor 1 (CAF1), a key factor involved in histone deposition, with the replication kinase Cdc7‐Dbf4. We isolated a complex containing both the largest subunit of CAF1 (p150) and the Cdc7‐Dbf4 kinase specifically in S phase and thus prove the existence of this interaction in vivo . We then show that the Cdc7‐Dbf4 kinase efficiently phosphorylates p150. This event induces a change in p150 oligomerization state, which promotes binding to proliferating cell nuclear antigen (PCNA). Conversely, CAF1 recruitment is reduced in a PCNA/DNA loading assay using Cdc7‐depleted extracts. Our data define p150 as a new target for this kinase with implications for the coordination between DNA replication and CAF1 functions.