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The chromatin remodelling complex WSTF–SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription
Author(s) -
Percipalle Piergiorgio,
Fomproix Nathalie,
Cavellán Erica,
Voit Renate,
Reimer Georg,
Krüger Tim,
Thyberg Johan,
Scheer Ulrich,
Grummt Ingrid,
Östlund Farrants AnnKristin
Publication year - 2006
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400657
Subject(s) - transcription (linguistics) , microbiology and biotechnology , rna polymerase ii , chromatin , polymerase , rna polymerase , biology , transcription factories , rna , genetics , gene , gene expression , promoter , philosophy , linguistics
Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein–protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference‐mediated knockdown of NM1 and WSTF reduced pre‐rRNA synthesis in vivo , and antibodies to WSTF inhibited Pol I transcription on pre‐assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.