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Type A and B RNase P RNAs are interchangeable in vivo despite substantial biophysical differences
Author(s) -
Wegscheid Barbara,
Condon Ciarán,
Hartmann Roland K
Publication year - 2006
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400641
Subject(s) - rnase p , ribonuclease , in vivo , rnase mrp , biology , ribonuclease iii , enzyme , biogenesis , function (biology) , transfer rna , rnase ph , rna , biochemistry , microbiology and biotechnology , genetics , gene , rna interference
We show that structural type A and B bacterial ribonuclease P (RNase P) RNAs can fully replace each other in vivo despite the many reported differences in their biogenesis, biochemical/biophysical properties and enzyme function in vitro . Our findings suggest that many of the reported idiosyncrasies of type A and B enzymes either do not reflect the in vivo situation or are not crucial for RNase P function in vivo , at least under standard growth conditions. The discrimination of mature tRNA by RNase P, so far thought to prevent product inhibition of the enzyme in the presence of a large cellular excess of mature tRNA relative to the precursor form, is apparently not crucial for RNase P function in vivo .