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Transcriptional regulation of the Neurospora circadian clock gene wc‐1 affects the phase of circadian output
Author(s) -
Káldi Krisztina,
González Beatriz Herreros,
Brunner Michael
Publication year - 2006
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400595
Subject(s) - biology , neurospora , circadian clock , circadian rhythm , conidiation , neurospora crassa , microbiology and biotechnology , transcription (linguistics) , promoter , genetics , gene expression , gene , endocrinology , mutant , linguistics , philosophy
WHITE COLLAR‐1 (WC‐1) is the limiting component of the White Collar Complex (WCC) controlling expression of the Neurospora circadian clock protein Frequency (FRQ). Accumulation of WC‐1 is supported by FRQ on a post‐transcriptional level. Here, we show that transcription of wc‐1 is organized in a complex way. Three promoters drive transcription of wc‐1 . P dist is dependent on WCC. P prox is independent of WCC in darkness, but inducible by light in a WCC‐dependent manner. A third promoter, P int , is located in the wc‐1 open reading frame and promotes expression of an amino‐terminally truncated WC‐1 isoform of unknown function. Expression of wc‐1 by P dist or P prox alone, or by a heterologous promoter, affects the entrained phase of circadian conidiation and the response of Neurospora to light. Our results indicate that transcriptional regulation of wc‐1 is required to modulate the circadian phase of clock output.