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Phosphorothioate‐stimulated uptake of short interfering RNA by human cells
Author(s) -
Overhoff Marita,
Sczakiel Georg
Publication year - 2005
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400535
Subject(s) - rna , small interfering rna , microbiology and biotechnology , biology , chemistry , biophysics , biochemistry , gene
The cellular delivery of short interfering RNA (siRNA) is a main hurdle in therapeutic drug development. Here, we describe that phosphorothioate (PTO)‐derived oligonucleotides stimulate the physical cellular uptake of siRNA in trans in human cells. This is reflected by an apparent dose‐dependent siRNA‐mediated suppression of lamin A/C in primary human umbilical vein endothelial cells. The PTO‐stimulated cellular uptake in trans is concentration dependent, length dependent, related to the phosphorothioate chemistry but not sequence specific. We provide experimental evidence to support a caveolin‐mediated uptake mechanism. In sum, this work strongly suggests the exploration of PTOs as facilitators in the delivery of biologically active siRNA to mammalian cells.