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TRBP, a regulator of cellular PKR and HIV‐1 virus expression, interacts with Dicer and functions in RNA silencing
Author(s) -
Haase Astrid D,
Jaskiewicz Lukasz,
Zhang Haidi,
Lainé Sébastien,
Sack Ragna,
Gatignol Anne,
Filipowicz Witold
Publication year - 2005
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400509
Subject(s) - dicer , argonaute , biology , small interfering rna , rna silencing , rna interference , gene silencing , rna induced silencing complex , microbiology and biotechnology , rna , effector , drosha , microrna , trans acting sirna , rna binding protein , protein kinase r , genetics , protein kinase a , kinase , gene , mitogen activated protein kinase kinase
Dicer is a key enzyme involved in RNA interference (RNAi) and microRNA (miRNA) pathways. It is required for biogenesis of miRNAs and small interfering RNAs (siRNAs), and also has a role in the effector steps of RNA silencing. Apart from Argonautes, no proteins are known to associate with Dicer in mammalian cells. In this work, we describe the ide.jpgication of TRBP (human immunodeficiency virus (HIV‐1) transactivating response (TAR) RNA‐binding protein) as a protein partner of human Dicer. We show that TRBP is required for optimal RNA silencing mediated by siRNAs and endogenous miRNAs, and that it facilitates cleavage of pre‐miRNA in vitro . TRBP had previously been assigned several functions, including inhibition of the interferon‐induced double‐stranded RNA‐regulated protein kinase PKR and modulation of HIV‐1 gene expression by association with TAR. The TRBP–Dicer interaction shown raises interesting questions about the potential interplay between RNAi and interferon–PKR pathways.