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The small chemical vacuolin‐1 alters the morphology of lysosomes without inhibiting Ca 2+ ‐regulated exocytosis
Author(s) -
Huynh Chau,
Andrews Norma W
Publication year - 2005
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400495
Subject(s) - exocytosis , lysosome , microbiology and biotechnology , membrane , chemistry , biology , biochemistry , biophysics , enzyme
Ca 2+ ‐regulated exocytosis of lysosomes was previously shown to be required for the repair of plasma membrane wounds. The small chemical vacuolin‐1 alters the morphology of lysosomes without affecting the ability of cells to reseal their plasma membrane after injury. On the basis of a failure to detect Ca 2+ ‐triggered lysosomal exocytosis in vacuolin‐1‐treated cells, a recent study proposed that lysosomes are dispensable for resealing. Here, we show that vacuolin‐1, despite altering lysosome morphology, does not inhibit the exocytosis of lysosomes induced by exposure to a Ca 2+ ionophore, or by plasma membrane wounding. Thus, lysosomes cannot be excluded as agents of membrane repair in vacuolin‐1‐treated cells.