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tRNA‐balanced expression of a eukaryal aminoacyl‐tRNA synthetase by an mRNA‐mediated pathway
Author(s) -
Frugier Magali,
Ryckelynck Michaël,
Giegé Richard
Publication year - 2005
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400481
Subject(s) - transfer rna , translation (biology) , aminoacylation , aminoacyl trna synthetase , messenger rna , rna , biology , gene , gene expression , amino acid , rna binding protein , protein biosynthesis , translational regulation , biochemistry
Aminoacylation of transfer RNAs is a key step during translation. It is catalysed by the aminoacyl‐tRNA synthetases (aaRSs) and requires the specific recognition of their cognate substrates, one or several tRNAs, ATP and the amino acid. Whereas the control of certain aaRS genes is well known in prokaryotes, little is known about the regulation of eukaryotic aaRS genes. Here, it is shown that expression of AspRS is regulated in yeast by a feedback mechanism that necessitates the binding of AspRS to its messenger RNA. This regulation leads to a synchronized expression of AspRS and tRNA Asp . The correlation between AspRS expression and mRNA AspRS and tRNA Asp concentrations, as well as the presence of AspRS in the nucleus, suggests an original regulation mechanism. It is proposed that the surplus of AspRS, not sequestered by tRNA Asp , is imported into the nucleus where it binds to mRNA AspRS and thus inhibits its accumulation.