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The structure of the TRAPP subunit TPC6 suggests a model for a TRAPP subcomplex
Author(s) -
Kümmel Daniel,
Müller Jürgen J,
Roske Yvette,
Misselwitz Rolf,
Büssow Konrad,
Heinemann Udo
Publication year - 2005
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400463
Subject(s) - protein subunit , microbiology and biotechnology , sequence (biology) , angstrom , dimer , structural similarity , chemistry , biology , biochemistry , crystallography , gene , organic chemistry
The TRAPP (transport protein particle) complexes are tethering complexes that have an important role at the different steps of vesicle transport. Recently, the crystal structures of the TRAPP subunits SEDL and BET3 have been determined, and we present here the 1.7 Å crystal structure of human TPC6, a third TRAPP subunit. The protein adopts an α/β‐plait topology and forms a dimer. In spite of low sequence similarity, the structure of TPC6 strikingly resembles that of BET3. The similarity is especially prominent at the dimerization interfaces of the proteins. This suggests heterodimerization of TPC6 and BET3, which is shown by in vitro and in vivo association studies. Together with TPC5, another TRAPP subunit, TPC6 and BET3 are supposed to constitute a family of paralogous proteins with closely similar three‐dimensional structures but little sequence similarity among its members.