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Glutamyl‐tRNA mediates a switch in RNA polymerase use during chloroplast biogenesis
Author(s) -
Hanaoka Mitsumasa,
Kanamaru Kengo,
Fujiwara Makoto,
Takahashi Hideo,
Tanaka Kan
Publication year - 2005
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400411
Subject(s) - plastid , rna polymerase , biology , rna polymerase iii , rna polymerase ii , transcription (linguistics) , rna editing , microbiology and biotechnology , rna , transfer rna , polymerase , rna polymerase i , gene , chloroplast , genetics , gene expression , promoter , linguistics , philosophy
Chloroplast genes of higher plants are transcribed by two types of RNA polymerase that are encoded by nuclear (NEP (nuclear‐encoded plastid RNA polymerase)) or plastid (PEP (plastid‐encoded plastid RNA polymerase)) genomes. NEP is largely responsible for the transcription of housekeeping genes during early chloroplast development. Subsequent light‐dependent chloroplast maturation is accompanied by repression of NEP activity and activation of PEP. Here, we show that the plastid‐encoded transfer RNA for glutamate, the expression of which is dependent on PEP, directly binds to and inhibits the transcriptional activity of NEP in vitro . The plastid tRNA Glu thus seems to mediate the switch in RNA polymerase usage from NEP to PEP during chloroplast development.