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The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants
Author(s) -
Flores Maria Jose,
Bidnenko Vladimir,
Michel Bénédicte
Publication year - 2004
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400262
Subject(s) - biology , dna replication , helicase , dna polymerase , replisome , genetics , dna repair , dnaa , control of chromosome duplication , polymerase , dna polymerase ii , recbcd , dna polymerase i , microbiology and biotechnology , circular bacterial chromosome , dna , gene , polymerase chain reaction , reverse transcriptase , rna
Replication forks arrested by inactivation of the main Escherichia coli DNA polymerase (polymerase III) are reversed by the annealing of newly synthesized leading‐ and lagging‐strand ends. Reversed forks are reset by the action of RecBC on the DNA double‐strand end, and in the absence of RecBC chromosomes are linearized by the Holliday junction resolvase RuvABC. We report here that the UvrD helicase is essential for RuvABC‐dependent chromosome linearization in E. coli polymerase III mutants, whereas its partners in DNA repair (UvrA/B and MutL/S) are not. We conclude that UvrD participates in replication fork reversal in E. coli .