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A neural‐specific splicing event generates an active form of the Wiskott‐Aldrich syndrome protein
Author(s) -
Le Page Yann,
Demay Florence,
Salbert Gilles
Publication year - 2004
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400239
Subject(s) - podosome , wiskott–aldrich syndrome protein , biology , microbiology and biotechnology , rna splicing , vinculin , actin , exon , rna , actin cytoskeleton , genetics , gene , cytoskeleton , signal transduction , cell , focal adhesion
Actin polymerization is required for cellular events such as podosome, lamellipode or filopode formation in migrating cells, and members of the Wiskott–Aldrich syndrome protein (WASP) family have essential roles in regulating actin dynamics at the cell leading edge. However, WASP proteins need first to be activated in order to be able to target actin polymerization. Here, we show the occurrence of a neural‐specific splicing event, which is favoured by the nuclear orphan receptor chicken ovalbumin upstream promoter–transcription factor I, and generates a truncated WASP protein deleted of exon 2‐encoded amino acids. This deletion relocates the protein to the plasma membrane and induces the formation of actin‐rich podosome‐like structures that also contain paxillin and vinculin. Furthermore, expression of the truncated protein in PC12 cells, as well as in primary neurons, stimulates neuritogenesis. These data underscore the importance of the neural‐specific splicing of WASP RNA during development.