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Slow Ca 2+ dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping
Author(s) -
Shimozono Satoshi,
Fukano Takashi,
Kimura Koutarou D,
Mori Ikue,
Kirino Yutaka,
Miyawaki Atsushi
Publication year - 2004
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400142
Subject(s) - peristalsis , calcium , caenorhabditis elegans , coupling (piping) , biophysics , fluorescence , excited state , chemistry , anatomy , biology , materials science , physics , biochemistry , optics , atomic physics , gene , organic chemistry , metallurgy
The pharyngeal muscles of Caenorhabditis elegans are composed of the corpus, isthmus and terminal bulb from anterior to posterior. These components are excited in a coordinated fashion to facilitate proper feeding through pumping and peristalsis. We analysed the spatiotemporal pattern of intracellular calcium dynamics in the pharyngeal muscles during feeding. We used a new ratiometric fluorescent calcium indicator and a new optical system that allows simultaneous illumination and detection at any two wavelengths. Pumping was observed with fast, repetitive and synchronous spikes in calcium concentrations in the corpus and terminal bulb, indicative of electrical coupling throughout the muscles. The posterior isthmus, however, responded to only one out of several pumping spikes to produce broad calcium transients, leading to peristalsis, the slow and gradual motion needed for efficient swallows. The excitation–calcium coupling may be uniquely modulated in this region at the level of calcium channels on the plasma membrane.