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Switching the mode of metabolism in the yeast Saccharomyces cerevisiae
Author(s) -
Otterstedt Karin,
Larsson Christer,
Bill Roslyn M,
Ståhlberg Anders,
Boles Eckhard,
Hohmann Stefan,
Gustafsson Lena
Publication year - 2004
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400132
Subject(s) - saccharomyces cerevisiae , yeast , biochemistry , biology , metabolism , hexose , carbohydrate metabolism , fermentation , cellular respiration , microbiology and biotechnology , mitochondrion , enzyme
The biochemistry of most metabolic pathways is conserved from bacteria to humans, although the control mechanisms are adapted to the needs of each cell type. Oxygen depletion commonly controls the switch from respiration to fermentation. However, Saccharomyces cerevisiae also controls that switch in response to the external glucose level. We have generated an S. cerevisiae strain in which glucose uptake is dependent on a chimeric hexose transporter mediating reduced sugar uptake. This strain shows a fully respiratory metabolism also at high glucose levels as seen for aerobic organisms, and switches to fermentation only when oxygen is lacking. These observations illustrate that manipulating a single step can alter the mode of metabolism. The novel yeast strain is an excellent tool to study the mechanisms underlying glucose‐induced signal transduction.