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The BAR‐domain family of proteins: a case of bending and binding?
Author(s) -
Habermann Bianca
Publication year - 2004
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400105
Subject(s) - amphiphysin , gtpase , sorting nexin , endocytosis , signal transducing adaptor protein , gtpase activating protein , microbiology and biotechnology , biology , endocytic cycle , computational biology , protein structure , genetics , endosome , intracellular , biochemistry , signal transduction , dynamin , receptor , g protein
BAR‐domains recently took centre stage in science through a report on the crystal structure of this domain in Drosophila Amphiphysin. Though only weakly conserved at the sequence level, the structure of the BAR domain shows striking similarity to the GTPase‐binding domain of Arfaptin 2, an effector of Rho‐ and Arf‐ GTPases. On the basis of this sequence and structural similarity, these two proteins have been classified as belonging to the same family, the BAR‐domain family, and they probably also have similar functional characteristics. Presented here are the results of a database search for the sequence of the BAR domain of Amphiphysin and Arfaptin 2. This search identified a variety of related proteins, most of which are involved in intracellular transport and especially in endocytosis. For example, the BAR‐domain family includes Endophilins, GTPase‐activating proteins of the Centaurinβ family and Oligophrenins, the adaptor proteins APPL1 and APPL2 that were recently shown to interact with the small GTPase Rab5, as well as members of the Sorting nexin family. On the basis of the structures of Amphiphysin and Arfaptin 2 and the cellular role of Amphiphysins in the early steps of endocytosis, the functions of the BAR domain have been defined as a dimerization motif and as sensing and inducing membrane curvature. However, data on Arfaptin 2 and now also on the Adaptor proteins APPL1 and 2 suggest that another function of the BAR domain is to bind to small GTPases.