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Cell‐cycle regulation of cohesin stability along fission yeast chromosomes
Author(s) -
Bernard Pascal,
Schmidt Christine Katrin,
Vaur Sabine,
Dheur Sonia,
Drogat Julie,
Genier Sylvie,
Ekwall Karl,
Uhlmann Frank,
Javerzat JeanPaul
Publication year - 2008
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601955
Subject(s) - cohesin , establishment of sister chromatid cohesion , biology , microbiology and biotechnology , chromatin , chromosome segregation , genetics , separase , dna , chromosome , gene
Sister chromatid cohesion is mediated by cohesin, but the process of cohesion establishment during S‐phase is still enigmatic. In mammalian cells, cohesin binding to chromatin is dynamic in G1, but becomes stabilized during S‐phase. Whether the regulation of cohesin stability is integral to the process of cohesion establishment is unknown. Here, we provide evidence that fission yeast cohesin also displays dynamic behavior. Cohesin association with G1 chromosomes requires continued activity of the cohesin loader Mis4/Ssl3, suggesting that repeated loading cycles maintain cohesin binding. Cohesin instability in G1 depends on wpl1 , the fission yeast ortholog of mammalian Wapl, suggestive of a conserved mechanism that controls cohesin stability on chromosomes. wpl1 is nonessential, indicating that a change in wpl1 ‐dependent cohesin dynamics is dispensable for cohesion establishment. Instead, we find that cohesin stability increases at the time of S‐phase in a reaction that can be uncoupled from DNA replication. Hence, cohesin stabilization might be a pre‐requisite for cohesion establishment rather than its consequence.