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14‐3‐3 Proteins recognize a histone code at histone H3 and are required for transcriptional activation
Author(s) -
Winter Stefan,
Simboeck Elisabeth,
Fischle Wolfgang,
Zupkovitz Gordin,
Dohnal Ilse,
Mechtler Karl,
Ammerer Gustav,
Seiser Christian
Publication year - 2008
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601954
Subject(s) - biology , histone code , histone , histone h3 , histone h2a , histone methyltransferase , genetics , histone h1 , sap30 , computational biology , histone octamer , microbiology and biotechnology , nucleosome , dna
Interphase phosphorylation of S10 at histone H3 is linked to transcriptional activation of a specific subset of mammalian genes like HDAC1. Recently, 14‐3‐3 proteins have been described as detectors for this phosphorylated histone H3 form. Here, we report that 14‐3‐3 binding is modulated by combinatorial modifications of histone H3. S10 phosphorylation is necessary for an interaction, but additional H3K9 or H3K14 acetylation increases the affinity of 14‐3‐3 for histone H3. Histone H3 phosphoacetylation occurs concomitant with K9 methylation in vivo , suggesting that histone phosphorylation and acetylation can synergize to overcome repressive histone methylation. Chromatin immunoprecipitation experiments reveal recruitment of 14‐3‐3 proteins to the HDAC1 gene in an H3S10ph‐dependent manner. Recruitment of 14‐3‐3 to the promoter is enhanced by additional histone H3 acetylation and correlates with dissociation of the repressive binding module HP1γ. Finally, siRNA‐mediated loss of 14‐3‐3 proteins abolishes the transcriptional activation of HDAC1. Together our data indicate that 14‐3‐3 proteins are crucial mediators of histone phosphoacetylation signals.