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SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC
Author(s) -
Kurshakova Maria M,
Krasnov Alexey N,
Kopytova Daria V,
Shidlovskii Yulii V,
Nikolenko Julia V,
Nabirochkina Ele,
Spehner Danièle,
Schultz Patrick,
Tora Làszlò,
Georgieva Sofia G
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601901
Subject(s) - transcription (linguistics) , gene knockdown , messenger rna , protein subunit , transcription factor , biology , microbiology and biotechnology , gene , nuclear export signal , cell nucleus , genetics , philosophy , linguistics
SAGA/TFTC‐type multiprotein complexes play important roles in the regulation of transcription. We have investigated the importance of the nuclear positioning of a gene, its transcription and the consequent export of the nascent mRNA. We show that E(y)2 is a subunit of the SAGA/TFTC‐type histone acetyl transferase complex in Drosophila and that E(y)2 concentrates at the nuclear periphery. We demonstrate an interaction between E(y)2 and the nuclear pore complex (NPC) and show that SAGA/TFTC also contacts the NPC at the nuclear periphery. E(y)2 forms also a complex with X ‐linked ma le s terile 2 (Xmas‐2) to regulate mRNA transport both in normal conditions and after heat shock. Importantly, E(y)2 and Xmas‐2 knockdown decreases the contact between the heat‐shock protein 70 ( hsp70) gene loci and the nuclear envelope before and after activation and interferes with transcription. Thus, E(y)2 and Xmas‐2 together with SAGA/TFTC function in the anchoring of a subset of transcription sites to the NPCs to achieve efficient transcription and mRNA export.