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Regulation of histone modification and cryptic transcription by the Bur1 and Paf1 complexes
Author(s) -
Chu Yaya,
Simic Rajna,
Warner Marcie H,
Arndt Karen M,
Prelich Gregory
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601887
Subject(s) - biology , histone , histone methylation , histone h3 , histone methyltransferase , acetylation , histone code , transcription (linguistics) , methylation , genetics , microbiology and biotechnology , gene , dna methylation , nucleosome , gene expression , linguistics , philosophy
The Bur1–Bur2 and Paf1 complexes function during transcription elongation and affect histone modifications. Here we describe new roles for Bur1–Bur2 and the Paf1 complex. We find that histone H3 K36 tri‐methylation requires specific components of the Paf1 complex and that K36 tri‐methylation is more strongly affected at the 5′ ends of genes in paf1 Δ and bur2 Δ strains in parallel with increased acetylation of histones H3 and H4. Interestingly, the 5′ increase in histone acetylation is independent of K36 methylation, and therefore is mechanistically distinct from the methylation‐driven deacetylation that occurs at the 3′ ends of genes. Finally, Bur1–Bur2 and the Paf1 complex have a second methylation‐independent function, since bur2 Δ set2 Δ and paf1 Δ set2 Δ double mutants display enhanced histone acetylation at the 3′ ends of genes and increased cryptic transcription initiation. These findings identify new functions for the Paf1 and Bur1–Bur2 complexes, provide evidence that histone modifications at the 5′ and 3′ ends of coding regions are regulated by distinct mechanisms, and reveal that the Bur1–Bur2 and Paf1 complexes repress cryptic transcription through a Set2‐independent pathway.