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The human DNA repair factor XPC‐HR23B distinguishes stereoisomeric benzo[ a ]pyrenyl‐DNA lesions
Author(s) -
Mocquet Vincent,
Kropachev Konstantin,
Kolbanovskiy Marina,
Kolbanovskiy Alexander,
Tapias Angels,
Cai Yuqin,
Broyde Suse,
Geacintov Nicholas E,
Egly JeanMarc
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601730
Subject(s) - biology , dna , dna repair , dna damage , microbiology and biotechnology , genetics
Benzo[ a ]pyrene (B[ a ]P), a known environmental pollutant and tobacco smoke carcinogen, is metabolically activated to highly tumorigenic B[ a ]P diol epoxide derivatives that predominantly form N 2 ‐guanine adducts in cellular DNA. Although nucleotide excision repair (NER) is an important cellular defense mechanism, the molecular basis of recognition of these bulky lesions is poorly understood. In order to investigate the effects of DNA adduct structure on NER, three stereoisomeric and conformationally different B[ a ]P‐ N 2 ‐dG lesions were site specifically incorporated into identical 135‐mer duplexes and their response to purified NER factors was investigated. Using a permanganate footprinting assay, the NER lesion recognition factor XPC/HR23B exhibits, in each case, remarkably different patterns of helix opening that is also markedly distinct in the case of an intra‐strand crosslinked cisplatin adduct. The different extents of helix distortions, as well as differences in the overall binding of XPC/HR23B to double‐stranded DNA containing either of the three stereoisomeric B[ a ]P‐ N 2 ‐dG lesions, are correlated with dual incisions catalyzed by a reconstituted incision system of six purified NER factors, and by the full NER apparatus in cell‐free nuclear extracts.