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The Golgi mitotic checkpoint is controlled by BARS‐dependent fission of the Golgi ribbon into separate stacks in G2
Author(s) -
Colanzi Antonino,
Carcedo Cristina Hidalgo,
Persico Angela,
Cericola Claudia,
Turacchio Gabriele,
Bonazzi Matteo,
Luini Alberto,
Corda Daniela
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601686
Subject(s) - biology , golgi apparatus , fission , mitosis , microbiology and biotechnology , ribbon , g2 m dna damage checkpoint , cell cycle checkpoint , genetics , cell , endoplasmic reticulum , cell cycle , physics , materials science , composite material , quantum mechanics , neutron
The Golgi ribbon is a complex structure of many stacks interconnected by tubules that undergo fragmentation during mitosis through a multistage process that allows correct Golgi inheritance. The fissioning protein CtBP1‐S/BARS (BARS) is essential for this, and is itself required for mitotic entry: a block in Golgi fragmentation results in cell‐cycle arrest in G2, defining the ‘Golgi mitotic checkpoint’. Here, we clarify the precise stage of Golgi fragmentation required for mitotic entry and the role of BARS in this process. Thus, during G2, the Golgi ribbon is converted into isolated stacks by fission of interstack connecting tubules. This requires BARS and is sufficient for G2/M transition. Cells without a Golgi ribbon are independent of BARS for Golgi fragmentation and mitotic entrance. Remarkably, fibroblasts from BARS‐knockout embryos have their Golgi complex divided into isolated stacks at all cell‐cycle stages, bypassing the need for BARS for Golgi fragmentation. This identifies the precise stage of Golgi fragmentation and the role of BARS in the Golgi mitotic checkpoint, setting the stage for molecular analysis of this process.