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The human Nup107–160 nuclear pore subcomplex contributes to proper kinetochore functions
Author(s) -
Zuccolo Michela,
Alves Annabelle,
Galy Vincent,
Bolhy Stéphanie,
Formstecher Etienne,
Racine Victor,
Sibarita JeanBaptiste,
Fukagawa Tatsuo,
Shiekhattar Ramin,
Yen Tim,
Doye Valérie
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601642
Subject(s) - biology , kinetochore , microbiology and biotechnology , centromere , nuclear pore , mitosis , genetics , nucleus , gene , chromosome
We previously demonstrated that a fraction of the human Nup107–160 nuclear pore subcomplex is recruited to kinetochores at the onset of mitosis. However, the molecular determinants for its kinetochore targeting and the functional significance of this localization were not investigated. Here, we show that the Nup107–160 complex interacts with CENP‐F, but that CENP‐F only moderately contributes to its targeting to kinetochores. In addition, we show that the recruitment of the Nup107–160 complex to kinetochores mainly depends on the Ndc80 complex. We further demonstrate that efficient depletion of the Nup107–160 complex from kinetochores, achieved either by combining siRNAs targeting several of its subunits excluding Seh1, or by depleting Seh1 alone, induces a mitotic delay. Further analysis of Seh1‐depleted cells revealed impaired chromosome congression, reduced kinetochore tension and kinetochore–microtubule attachment defects. Finally, we show that the presence of the Nup107–160 complex at kinetochores is required for the recruitment of Crm1 and RanGAP1–RanBP2 to these structures. Together, our data thus provide the first molecular clues underlying the function of the human Nup107–160 complex at kinetochores.