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Characterization of GATA‐1 + hemangioblastic cells in the mouse embryo
Author(s) -
Yokomizo Tomomasa,
Takahashi Satoru,
Mochizuki Naomi,
Kuroha Takashi,
Ema Masatsugu,
Wakamatsu Asami,
Shimizu Ritsuko,
Ohneda Osamu,
Osato Motomi,
Okada Hitoshi,
Komori Toshihisa,
Ogawa Minetaro,
Nishikawa ShinIchi,
Ito Yoshiaki,
Yamamoto Masayuki
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601480
Subject(s) - biology , embryo , microbiology and biotechnology , genetics
Hemangioblasts are thought to be one of the sources of hematopoietic progenitors, yet little is known about their localization and fate in the mouse embryo. We show here that a subset of cells co‐expressing the hematopoietic marker GATA‐1 and the endothelial marker VE‐cadherin localize on the yolk sac blood islands at embryonic day 7.5. Clonal analysis demonstrated that GATA‐1 + cells isolated from E7.0–7.5 embryos include a common precursor for hematopoietic and endothelial cells. Moreover, this precursor possesses primitive and definitive hematopoietic bipotential. By using a transgenic complementation rescue approach, GATA‐1 + cell‐derived progenitors were selectively restored in Runx1‐deficient mice. In the rescued mice, definitive erythropoiesis was recovered but the rescued progenitors did not display multilineage hematopoiesis or intra‐aortic hematopoietic clusters. These results provide evidence of the presence of GATA‐1 + hemangioblastic cells in the extra‐embryonic region and also their functional contribution to hematopoiesis in the embryo.