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The human synMuv‐like protein LIN‐9 is required for transcription of G2/M genes and for entry into mitosis
Author(s) -
Osterloh Lisa,
von Eyss Björn,
Schmit Fabienne,
Rein Lena,
Hübner Denise,
Samans Birgit,
Hauser Stefanie,
Gaubatz Stefan
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601478
Subject(s) - biology , mitosis , transcription (linguistics) , gene , genetics , microbiology and biotechnology , philosophy , linguistics
Regulated gene expression is critical for the proper timing of cell cycle transitions. Here we report that human LIN‐9 has an important function in transcriptional regulation of G2/M genes. Depletion of LIN‐9 by RNAi in human fibroblasts strongly impairs proliferation and delays progression from G2 to M. We identify a cluster of G2/M genes as direct targets of LIN‐9. Activation of these genes is linked to an association between LIN‐9 and B‐MYB. Chromatin immunoprecipitation assays revealed binding of both LIN‐9 and B‐MYB to the promoters of G2/M regulated genes. Depletion of B‐MYB recapitulated the biological outcome of LIN‐9 knockdown, including impaired proliferation and reduced expression of G2/M genes. These data suggest a critical role for human LIN‐9, together with B‐MYB, in the activation of genes that are essential for progression into mitosis.