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Palmitoylation is required for efficient Fas cell death signaling
Author(s) -
Chakrabandhu Krittalak,
Hérincs Zoltán,
Huault Sébastien,
Dost Britta,
Peng Ling,
Conchonaud Fabien,
Marguet Didier,
He HaiTao,
Hueber AnneOdile
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601456
Subject(s) - palmitoylation , microbiology and biotechnology , lipid raft , biology , programmed cell death , signal transduction , cytoskeleton , fas ligand , internalization , actin cytoskeleton , ezrin , fas receptor , caspase , apoptosis , cell , biochemistry , cysteine , enzyme
Localization of the death receptor Fas to specialized membrane microdomains is crucial to Fas‐mediated cell death signaling. Here, we report that the post‐translational modification of Fas by palmitoylation at the membrane proximal cysteine residue in the cytoplasmic region is the targeting signal for Fas localization to lipid rafts, as demonstrated in both cell‐free and living cell systems. Palmitoylation is required for the redistribution of Fas to actin cytoskeleton‐linked rafts upon Fas stimulation and for the raft‐dependent, ezrin‐mediated cytoskeleton association, which is necessary for the efficient Fas receptor internalization, death‐inducing signaling complex assembly and subsequent caspase cascade leading to cell death.