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Cargo selection by specific kinesin light chain 1 isoforms
Author(s) -
Woźniak Marcin J,
Allan Victoria J
Publication year - 2006
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601427
Subject(s) - biology , kinesin , gene isoform , selection (genetic algorithm) , immunoglobulin light chain , computational biology , microbiology and biotechnology , genetics , microtubule , gene , artificial intelligence , computer science , antibody
Kinesin‐1 drives the movement of diverse cargoes, and it has been proposed that specific kinesin light chain (KLC) isoforms target kinesin‐1 to these different structures. Here, we test this hypothesis using two in vitro motility assays, which reconstitute the movement of rough endoplasmic reticulum (RER) and vesicles present in a Golgi membrane fraction. We generated GST‐tagged fusion proteins of KLC1B and KLC1D that included the tetratricopeptide repeat domain and the variable C‐terminus. We find that preincubation of RER with KLC1B inhibits RER motility, whereas KLC1D does not. In contrast, Golgi fraction vesicle movement is inhibited by KLC1D but not KLC1B reagents. Both RER and vesicle movement is inhibited by preincubation with the GST‐tagged C‐terminal domain of ubiquitous kinesin heavy chain (uKHC), which binds to the N‐terminal domain of uKHC and alters its interaction with microtubules. We propose that although the TRR domains are required for cargo binding, it is the variable C‐terminal region of KLCs that are vital for targeting kinesin‐1 to different cellular structures.