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Rad52‐mediated DNA annealing after Rad51‐mediated DNA strand exchange promotes second ssDNA capture
Author(s) -
Sugiyama Tomohiko,
Kantake Noriko,
Wu Yun,
Kowalczykowski Stephen C
Publication year - 2006
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601412
Subject(s) - rad52 , rad51 , replication protein a , homologous recombination , biology , dna , dna repair , microbiology and biotechnology , dna clamp , biophysics , dna binding protein , genetics , transcription factor , rna , gene , reverse transcriptase
Rad51, Rad52, and RPA play central roles in homologous DNA recombination. Rad51 mediates DNA strand exchange, a key reaction in DNA recombination. Rad52 has two distinct activities: to recruit Rad51 onto single‐strand (ss)DNA that is complexed with the ssDNA‐binding protein, RPA, and to anneal complementary ssDNA complexed with RPA. Here, we report that Rad52 promotes annealing of the ssDNA strand that is displaced by DNA strand exchange by Rad51 and RPA, to a second ssDNA strand. An RPA that is recombination‐deficient (RPA(rfa1‐t11)) failed to support annealing, explaining its in vivo phenotype. Escherichia coli RecO and SSB proteins, which are functional homologues of Rad52 and RPA, also facilitated the same reaction, demonstrating its conserved nature. We also demonstrate that the two activities of Rad52, recruiting Rad51 and annealing DNA, are coordinated in DNA strand exchange and second ssDNA capture.