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Retracted: Regulation of MBD1‐mediated transcriptional repression by SUMO and PIAS proteins
Author(s) -
Lyst Matthew J,
Nan Xinsheng,
Stancheva Irina
Publication year - 2006
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601404
Subject(s) - research centre , library science , biology , computer science
In mammalian cells, DNA methylation is associated with heritable and stable gene repression, mediated in part by methyl‐CpG‐binding domain (MBD) proteins that recruit corepressors to modify chromatin. MBD1 protein, a member of the MBD family, forms a complex with SETDB1 histone methylase to silence transcription at target promoters by methylation of lysine 9 of histone H3. How MBD1‐mediated transcriptional repression is regulated is currently unknown. Here we show that MBD1 is a target for sumoylation by PIAS1 ( P rotein I nhibitors of A ctivated S TAT 1) and PIAS3 E3 SUMO ( s mall u biquitin‐like mo difier)‐ligases, at two conserved lysine residues within the C‐terminus of MBD1. Although sumoylated MBD1 binds to methylated DNA, it does not incorporate into a complex with SETDB1 and does not efficiently repress transcription of a target gene, p53BP2, in HeLa cells. Our data suggest that transcriptional silencing by MBD1 is regulated by a PIAS‐mediated conjugation of SUMO1, which antagonizes the formation of a repressive complex with SETDB1.