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Genome‐wide characterization of fission yeast DNA replication origins
Author(s) -
Heichinger Christian,
Penkett Christopher J,
Bähler Jürg,
Nurse Paul
Publication year - 2006
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601390
Subject(s) - biology , schizosaccharomyces pombe , mitosis , control of chromosome duplication , dna replication , genetics , schizosaccharomyces , genome , origin of replication , meiosis , dna re replication , eukaryotic dna replication , origin recognition complex , licensing factor , pre replication complex , saccharomyces cerevisiae , dna , yeast , gene
Eukaryotic DNA replication is initiated from multiple origins of replication, but little is known about the global regulation of origins throughout the genome or in different types of cell cycles. Here, we identify 401 strong origins and 503 putative weaker origins spaced in total every 14 kb throughout the genome of the fission yeast Schizosaccharomyces pombe . The same origins are used during premeiotic and mitotic S‐phases. We found that few origins fire late in mitotic S‐phase and that activating the Rad3 dependent S‐phase checkpoint by inhibiting DNA replication had little effect on which origins were fired. A genome‐wide analysis of eukaryotic origin efficiencies showed that efficiency was variable, with large chromosomal domains enriched for efficient or inefficient origins. Average efficiency is twice as high during mitosis compared with meiosis, which can account for their different S‐phase lengths. We conclude that there is a continuum of origin efficiency and that there is differential origin activity in the mitotic and meiotic cell cycles.