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Early presynaptic changes during plasticity in cultured hippocampal neurons
Author(s) -
Ninan Ipe,
Liu Shumin,
Rabinowitz Daniel,
Arancio Ottavio
Publication year - 2006
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601318
Subject(s) - columbia university , library science , history , sociology , media studies , computer science
Long‐lasting increase in synaptic strength is thought to underlie learning. An explosion of data has characterized changes in postsynaptic (pstS) AMPA receptor cycling during potentiation. However, changes occurring within the presynaptic (prS) terminal remain largely unknown. We show that appearance of new release sites during potentiation between cultured hippocampal neurons is due to (a) conversion of nonrecycling sites to recycling sites, (b) formation of new releasing sites from areas containing diffuse staining for the prS marker Vesicle‐Associated Membrane Protein‐2 and (c) budding of new recycling sites from previously existing recycling sites. In addition, potentiation is accompanied by a release probability increase in pre‐existing boutons depending upon their individual probability. These prS changes precede and regulate fluorescence increase for pstS GFP‐tagged‐AMPA‐receptor subunit GluR1. These results suggest that potentiation involves early changes in the prS terminal including remodeling and release probability increase of pre‐existing synapses.