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Lineage‐specific activators affect β‐globin locus chromatin in multipotent hematopoietic progenitors
Author(s) -
Bottardi Stefania,
Ross Julie,
PierreCharles Natacha,
Blank Volker,
Milot Eric
Publication year - 2006
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601232
Subject(s) - biology , chromatin , globin , progenitor cell , haematopoiesis , genetics , locus (genetics) , multipotent stem cell , microbiology and biotechnology , lineage (genetic) , stem cell , gene
During development, the regulated expression of tissue‐specific genes can be preceded by their potentiation, that is, by chromatin activation in progenitor cells. For example, the human β‐like globin genes are potentiated in a gene‐ and developmental‐specific manner in hematopoietic progenitors. Developmental regulation of human β‐gene expression in erythroid cells is mostly determined by transcriptional activators; however, it is not clear how gene‐specific potentiation is set in hematopoietic progenitors. Using human and transgenic multipotent hematopoietic progenitors, we demonstrate that human β‐globin locus activation is characterized by TBP, NF‐E2, CBP and BRG1 recruitment at both the Locus Control Region and human β‐gene promoter. Our results further indicate that in hematopoietic progenitors, EKLF influences chromatin organization at the human β‐globin locus and is instrumental for human β‐gene potentiation. Thus, we show that lineage‐specific transcriptional activators expressed at basal levels in progenitor cells can participate in gene potentiation.