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Transcription regulation from a TATA and INR‐less promoter: spatial segregation of promoter function
Author(s) -
George Anuja A,
Sharma Manish,
Singh Badri N,
Sahoo Naresh C,
Rao Kanury VS
Publication year - 2006
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7600966
Subject(s) - biology , tata box , transcription (linguistics) , promoter , genetics , transcription factor , computational biology , gene , gene expression , linguistics , philosophy
The mode of regulation of class II genes that lack the known core promoter elements is presently unclear. Here, we studied one such example, the murine CD80 gene. An unusual mechanism was revealed wherein the pre‐initiation complex (PIC) first assembled on an upstream, NF‐κB enhancer element. Notably, this assembly occurred independent of contributions from the core promoter domain, and resulted in a PIC that was competent for transcription initiation. Positioning was subsequently achieved by exploiting the intrinsic architecture of the promoter, by virtue of which the tethered PIC was spatially juxtaposed with the transcription initiation site. Bridging interactions then ensued, through protein–protein contacts, which then enabled the elongation phase of CD80 transcription.