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A role for BARS at the fission step of COPI vesicle formation from Golgi membrane
Author(s) -
Yang JiaShu,
Lee Stella Y,
Spanò Stefania,
Gad Helge,
Zhang Leiliang,
Nie Zhongzhen,
Bonazzi Matteo,
Corda Daniela,
Luini Alberto,
Hsu Victor W
Publication year - 2005
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7600873
Subject(s) - copi , biology , golgi apparatus , vesicle , fission , microbiology and biotechnology , biophysics , membrane , endoplasmic reticulum , secretory pathway , biochemistry , physics , nuclear physics , neutron
The core complex of Coat Protein I (COPI), known as coatomer, is sufficient to induce coated vesicular‐like structures from liposomal membrane. In the context of biological Golgi membrane, both palmitoyl‐coenzyme A (p‐coA) and ARFGAP1, a GTPase‐activating protein (GAP) for ADP‐Ribosylation Factor 1, also participate in vesicle formation, but how their roles may be linked remains unknown. Moreover, whether COPI vesicle formation from Golgi membrane requires additional factors also remains unclear. We now show that Brefeldin‐A ADP‐Ribosylated Substrate (BARS) plays a critical role in the fission step of COPI vesicle formation from Golgi membrane. This role of BARS requires its interaction with ARFGAP1, which is in turn regulated oppositely by p‐coA and nicotinamide adenine dinucleotide, which act as cofactors of BARS. Our findings not only identify a new factor needed for COPI vesicle formation from Golgi membrane but also reveal a surprising mechanism by which the roles of p‐coA and GAP are linked in this process.