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Nup50/Npap60 function in nuclear protein import complex disassembly and importin recycling
Author(s) -
Matsuura Yoshiyuki,
Stewart Murray
Publication year - 2005
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7600843
Subject(s) - importin , nuclear transport , nls , nuclear localization sequence , biology , nuclear pore , ran , nucleoporin , microbiology and biotechnology , karyopherin , nuclear protein , cytoplasm , cell nucleus , biochemistry , transcription factor , gene
Nuclear import of proteins containing classical nuclear localization signals (NLS) is mediated by the importin‐α:β complex that binds cargo in the cytoplasm and facilitates its passage through nuclear pores, after which nuclear RanGTP dissociates the import complex and the importins are recycled. In vertebrates, import is stimulated by nucleoporin Nup50, which has been proposed to accompany the import complex through nuclear pores. However, we show here that the Nup50 N‐terminal domain actively displaces NLSs from importin‐α, which would be more consistent with Nup50 functioning to coordinate import complex disassembly and importin recycling. The crystal structure of the importin‐α:Nup50 complex shows that Nup50 binds at two sites on importin‐α. One site overlaps the secondary NLS‐binding site, whereas the second extends along the importin‐α C‐terminus. Mutagenesis indicates that interaction at both sites is required for Nup50 to displace NLSs. The Cse1p:Kap60p:RanGTP complex structure suggests how Nup50 is then displaced on formation of the importin‐α export complex. These results provide a rationale for understanding the series of interactions that orchestrate the terminal steps of nuclear protein import.