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v‐SNAREs control exocytosis of vesicles from priming to fusion
Author(s) -
Borisovska Maria,
Zhao Ying,
Tsytsyura Yaroslav,
Glyvuk Nataliya,
Takamori Shigeo,
Matti Ulf,
Rettig Jens,
Südhof Thomas,
Bruns Dieter
Publication year - 2005
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7600696
Subject(s) - physiology , library science , medicine , computer science
SNARE proteins (soluble NSF‐attachment protein receptors) are thought to be central components of the exocytotic mechanism in neurosecretory cells, but their precise function remained unclear. Here, we show that each of the vesicle‐associated SNARE proteins (v‐SNARE) of a chromaffin granule, synaptobrevin II or cellubrevin, is sufficient to support Ca 2+ ‐dependent exocytosis and to establish a pool of primed, readily releasable vesicles. In the absence of both proteins, secretion is abolished, without affecting biogenesis or docking of granules indicating that v‐SNAREs are absolutely required for granule exocytosis. We find that synaptobrevin II and cellubrevin differentially control the pool of readily releasable vesicles and show that the v‐SNARE's amino terminus regulates the vesicle's primed state. We demonstrate that dynamics of fusion pore dilation are regulated by v‐SNAREs, indicating their action throughout exocytosis from priming to fusion of vesicles.