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Functional link between ribosome formation and biogenesis of iron–sulfur proteins
Author(s) -
Yarunin Alexander,
Panse Vikram Govind,
Petfalski Elisabeth,
Dez Christophe,
Tollervey David,
Hurt Eduard C
Publication year - 2005
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7600540
Subject(s) - library science , classics , history , computer science
In genetic screens for ribosomal export mutants, we identified CFD1 , NBP35 and NAR1 as factors involved in ribosome biogenesis. Notably, these components were recently reported to function in extramitochondrial iron–sulfur (Fe–S) cluster biosynthesis. In particular, Nar1 was implicated to generate the Fe–S clusters within Rli1, a potential substrate protein of unknown function. We tested whether the Fe–S protein Rli1 functions in ribosome formation. We report that rli1 mutants are impaired in pre‐rRNA processing and defective in the export of both ribosomal subunits. In addition, Rli1p is associated with both pre‐40S particles and mature 40S subunits, and with the eIF3 translation initiation factor complex. Our data reveal an unexpected link between ribosome biogenesis and the biosynthetic pathway of cytoplasmic Fe–S proteins.