Premium
Coupling between snoRNP assembly and 3′ processing controls box C/D snoRNA biosynthesis in yeast
Author(s) -
Morlando Mariangela,
Ballarino Monica,
Greco Paolo,
Caffarelli Elisa,
Dichtl Bernhard,
Bozzoni Irene
Publication year - 2004
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7600254
Subject(s) - small nucleolar rna , polyadenylation , biology , transcription (linguistics) , microbiology and biotechnology , cleavage (geology) , rna , rna polymerase ii , genetics , gene , promoter , long non coding rna , gene expression , paleontology , linguistics , philosophy , fracture (geology)
RNA polymerase II transcribes genes encoding proteins and a large number of small stable RNAs. While pre‐mRNA 3′‐end formation requires a machinery ensuring tight coupling between cleavage and polyadenylation, small RNAs utilize polyadenylation‐independent pathways. In yeast, specific factors required for snRNA and snoRNA 3′‐end formation were characterized as components of the APT complex that is associated with the core complex of the cleavage/polyadenylation machinery (core‐CPF). Other essential factors were identified as independent components: Nrd1p, Nab3p and Sen1p. Here we report that mutations in the conserved box D of snoRNAs and in the snoRNP‐specific factor Nop1p interfere with transcription and 3′‐end formation of box C/D snoRNAs. We demonstrate that Nop1p is associated with box C/D snoRNA genes and that it interacts with APT components. These data suggest a mechanism of quality control in which efficient transcription and 3′‐end formation occur only when nascent snoRNAs are successfully assembled into functional particles.