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Tyrosine phosphorylation controls Runx2‐mediated subnuclear targeting of YAP to repress transcription
Author(s) -
Zaidi Sayyed K,
Sullivan Andrew J,
Medina Ricardo,
Ito Yoshiaki,
van Wijnen Andre J,
Stein Janet L,
Lian Jane B,
Stein Gary S
Publication year - 2004
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7600073
Subject(s) - runx2 , biology , proto oncogene tyrosine protein kinase src , phosphorylation , tyrosine phosphorylation , transcription factor , microbiology and biotechnology , tyrosine kinase , signal transduction , gene , biochemistry
Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes‐associated protein (YAP), a mediator of Src/Yes signaling, interacts with the native Runx2 protein, an osteoblast‐related transcription factor, and suppresses Runx2 transcriptional activity in a dose‐dependent manner. Runx2, through its PY motif, recruits YAP to subnuclear domains in situ and to the osteocalcin (OC) gene promoter in vivo . Inhibition of Src/Yes kinase blocks tyrosine phosphorylation of YAP and dissociates endogenous Runx2–YAP complexes. Consequently, recruitment of the YAP co‐repressor to subnuclear domains is abrogated and expression of the endogenous OC gene is induced. Our results suggest that Src/Yes signals are integrated through organization of Runx2–YAP transcriptional complexes at subnuclear sites to attenuate skeletal gene expression.