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Roles of SWI/SNF and HATs throughout the dynamic transcription of a yeast glucose‐repressible gene
Author(s) -
Geng Fuqiang,
Laurent Brehon C
Publication year - 2004
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7600035
Subject(s) - biology , swi/snf , chromatin , chromatin remodeling , transcription (linguistics) , transcription factor , chromatin structure remodeling (rsc) complex , promoter , transcription coregulator , e box , genetics , microbiology and biotechnology , gene expression , histone , histone acetyltransferases , gene , regulation of gene expression , linguistics , philosophy
Eucaryotic gene expression requires chromatin‐remodeling activities. We show by time‐course studies that transcriptional induction of the yeast glucose‐regulated SUC2 gene is rapid and shows a striking biphasic pattern, the first phase of which is partly mediated by the general stress transcription factors Msn2p/Msn4p. The SWI/SNF ATP‐dependent chromatin‐remodeling complex associates with the promoter in a similar biphasic manner and is essential for both phases of transcription. Two different histone acetyltransferases, Gcn5p and Esa1p, enhance the binding of SWI/SNF to the promoter during early transcription and are required for optimal SUC2 induction. Gcn5p is recruited to SUC2 simultaneously with SWI/SNF, whereas Esa1p associates constitutively with the promoter. This study reveals an unusual transcription pattern of a metabolic gene and suggests a novel strategy by which distinct chromatin remodelers cooperate for the dynamic activation of transcription.