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Neuroprotective effect of hydrogen peroxide on an in vitro model of brain ischaemia
Author(s) -
Nisticò R,
Piccirilli S,
Cucchiaroni M L,
Armogida M,
Guatteo E,
Giampà C,
Fusco F R,
Bernardi G,
Nisticò G,
Mercuri N B
Publication year - 2008
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0707587
Subject(s) - neuroprotection , catalase , reactive oxygen species , superoxide dismutase , pharmacology , chemistry , excitatory postsynaptic potential , endogeny , hippocampal formation , biochemistry , slice preparation , hydrogen peroxide , oxidative stress , biophysics , biology , in vitro , neuroscience , receptor
Background and purpose: Reactive oxygen species (ROS) have been postulated to play a crucial role in the pathogenesis of ischaemia‐reperfusion injury. Among these, hydrogen peroxide (H 2 O 2 ) is known to be a toxic compound responsible for free‐radical‐dependent neuronal damage. In recent years, however, the ‘bad reputation’ of H 2 O 2 and other ROS molecules has changed. The aim of this study was to assess the protective role of H 2 O 2 and modification in its endogenous production on the electrophysiological and morphological changes induced by oxygen/glucose deprivation (OGD) on CA1 hippocampal neurons. Experimental approach: Neuroprotective effects of exogenous and endogenous H 2 O 2 were determined using extracellular electrophysiological recordings of field excitatory post synaptic potentials (fEPSPs) and morphological studies in a hippocampal slice preparation. In vitro OGD was delivered by switching to an artificial cerebrospinal fluid solution with no glucose and with oxygen replaced by nitrogen. Key results: Neuroprotection against in vitro OGD was observed in slices treated with H 2 O 2 (3 mM). The rescuing action of H 2 O 2 was mediated by catalase as pre‐treatment with the catalase inhibitor 3‐amino‐1,2,4‐triazole blocked this effect. More interestingly, we showed that an increase of the endogenous levels of H 2 O 2 , due to a combination of an inhibitor of the glutathione peroxidase enzyme and addition of Cu,Zn‐superoxide dismutase in the tissue bath, prevented the OGD‐induced irreversible depression of fEPSPs. Conclusions and implications: Taken together, our results suggest new possible strategies to lessen the damage produced by a transient brain ischaemia by increasing the endogenous tissue level of H 2 O 2 . British Journal of Pharmacology (2008) 153 , 1022–1029; doi: 10.1038/sj.bjp.0707587 ; published online 28 January 2008