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Thromboxane A 2 inhibition of SK Ca after NO synthase block in rat middle cerebral artery
Author(s) -
McNeish A J,
Garland C J
Publication year - 2007
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0707240
Subject(s) - apamin , iberiotoxin , hyperpolarization (physics) , cerebral arteries , myograph , thromboxane , endocrinology , thromboxane receptor , medicine , chemistry , thromboxane a2 , thromboxane a synthase , middle cerebral artery , endothelium derived hyperpolarizing factor , potassium channel , agonist , pharmacology , receptor , contraction (grammar) , charybdotoxin , ischemia , platelet , stereochemistry , nuclear magnetic resonance spectroscopy
Background and purpose: NO/prostanoid independent, EDHF‐mediated hyperpolarization and dilation in rat middle cerebral arteries is mediated solely by endothelial cell IK Ca . However, when the NO‐pathway is also active, both SK Ca and IK Ca contribute to EDHF responses. As the SK Ca component can be inhibited by stimulation of thromboxane A 2 (TxA 2 ) TP receptors and NO has the potential ability to inhibit thromboxane synthesis, we investigated whether TxA 2 might explain loss of functional input from SK Ca during NOS inhibition in cerebral arteries. Experimental approach: Rat middle cerebral arteries were mounted in a wire myograph. Endothelium‐dependent responses to the PAR2 agonist, SLIGRL were assessed as simultaneous changes in smooth muscle membrane potential and tension. Key results: Responses were obtained in the presence of L ‐NAME as appropriate. Inhibition of TP receptors with either ICI 192,605 or SQ 29,548, did not affect EDHF mediated hyperpolarization and relaxation, but in their presence neither TRAM‐34 nor apamin (to block IK Ca and SK Ca respectively) individually affected the EDHF response. However, in combination they virtually abolished it. Similar effects were obtained in the presence of the thromboxane synthase inhibitor, furegrelate, which additionally revealed an iberiotoxin‐sensitive residual EDHF hyperpolarization and relaxation in the combined presence of TRAM‐34 and apamin. Conclusions and implications: In the rat middle cerebral artery, inhibition of NOS leads to a loss of the SK Ca component of EDHF responses. Either antagonism of TP receptors or block of thromboxane synthase restores an input through SK Ca . These data indicate that NO normally enables SK Ca activity in rat middle cerebral arteries. British Journal of Pharmacology (2007) 151 , 441–449; doi: 10.1038/sj.bjp.0707240