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Inflammatory events in a vascular remodeling model induced by surgical injury to the rat carotid artery
Author(s) -
Rinaldi Barbara,
Romagnoli Paolo,
Bacci Stefano,
Carnuccio Rosa,
Maiuri Maria Chiara,
Donniacuo Maria,
Capuano Annalisa,
Rossi Francesco,
Filippelli Amelia
Publication year - 2006
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0706472
Subject(s) - neointima , western blot , common carotid artery , heat shock protein , hsp70 , pathology , medicine , chemistry , anatomy , carotid arteries , restenosis , biochemistry , stent , gene
The aim of our study was to gain insight into the molecular and cellular mechanisms of the inflammatory response to arterial injury in a rat experimental model. Rats (five for each experimental time) were subjected to brief clamping and longitudinal incision of a carotid artery and monitored for 30 days. Subsequently, Nuclear Factor‐kappaB (NF‐ κ B) expression was measured by electrophoretic mobility shift assay. Heat shock protein (HSP) 27, HSP47 and HSP70 were evaluated by Western blot. Morphological changes of the vessel wall were investigated by light and electron microscopy. In injured rat carotid artery NF‐ κ B activity started immediately upon injury, and peaked between 2 and 3 weeks later. Western blot showed a significant increase of HSP47 and HSP70 7 days after injury. At 2 weeks postinjury, HSP27 expression peaked. Ligth microscopy showed a neointima formation, discontinuity of the media layer and a rich infiltrate. Among infiltrating cells electron microscopy identified dendritic‐like cells in contact with lymphocytes. Our model of surgical injury induces a significant inflammatory process characterized by enhanced NF‐ κ B activity and HSPs hyperexpression. Dendritic‐like cells were for the first time identified as a novel component of tissue repair consequent to acute arterial injury.British Journal of Pharmacology (2006) 147 , 175–182. doi: 10.1038/sj.bjp.0706472