Nongenomic responses to 17 β ‐estradiol in male rat mesenteric arteries abolish intrinsic gender differences in vascular responses

The aim of the present study was to investigate the gender differences in the acute effects of 17 β ‐estradiol on the rat superior mesenteric artery. Isometric tension was measured in rings of mesenteric arteries from both male and female Sprague–Dawley rats. Relaxation to acetylcholine was not significantly different between arteries (with endothelium) from male and female rats in the absence or presence of 17 β ‐estradiol. After blockade of endothelium‐dependent hyperpolarizations with apamin (0.3  μ M ) plus charybdotoxin (0.1  μ M ), acute exposure to 17 β ‐estradiol (1 n M ) for 30 min resulted in enhancement of relaxation to acetylcholine in arteries from male but not female rats. After acute exposure to 17 β ‐estradiol, mesenteric arteries from male rats were more sensitive to sodium nitroprusside than arteries from female rats. Contractions of mesenteric arteries to phenylephrine and 9,11‐dideoxy‐11 α ,9 α ‐epoxymethanoprostaglandin F 2 α (U46619) were greater in arteries from male rats than female rats. This difference was not detected after acute exposure to 17 β ‐estradiol. In preparations without endothelium, the enhancement of relaxation and reduction in contraction in arteries from male rats were preserved. These results suggest that there exists a gender difference in the response to the acute nongenomic modulatory effect of 17 β ‐estradiol in rat mesenteric arteries. Arteries from male rats seem to be more sensitive to the modulatory effects of 17 β ‐estradiol than arteries from female rats. The effect appears to be mainly at the level of the vascular smooth muscles.British Journal of Pharmacology (2005) 146 , 1148–1155. doi: 10.1038/sj.bjp.0706422