Premium
Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells
Author(s) -
Kempuraj Duraisamy,
Madhappan Bhuvaneshwari,
Christodoulou Spyridon,
Boucher William,
Cao Jing,
Papadopoulou Nikoletta,
Cetrulo Curtis L,
Theoharides Theoharis C
Publication year - 2005
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0706246
Subject(s) - myricetin , proinflammatory cytokine , flavonols , morin , histamine , chemistry , kaempferol , quercetin , pharmacology , mast cell , biochemistry , inflammation , biology , immunology , medicine , antioxidant , pathology
1 Mast cells participate in allergies, and also in immunity and inflammation by secreting proinflammatory cytokines. 2 Flavonoids are naturally occurring polyphenolic plant compounds, one group of which – the flavonols, inhibits histamine and some cytokine release from rodent basophils and mast cells. However, the effect of flavonols on proinflammatory mediator release and their possible mechanism of action in human mast cells is not well defined. 3 Human umbilical cord blood‐derived cultured mast cells (hCBMCs) grown in the presence of stem cell factor (SCF) and interleukin (IL)‐6 were preincubated for 15 min with the flavonols quercetin, kaempferol, myricetin and morin (0.01, 0.1, 1, 10 or 100 μ M ), followed by activation with anti‐IgE. Secretion was quantitated for IL‐6, IL‐8, tumor necrosis factor‐alpha (TNF‐ α ), histamine and tryptase levels. 4 Release of IL‐6, IL‐8 and TNF‐ α was inhibited by 82–93% at 100 μ M quercetin and kaempferol, and 31–70% by myricetin and morin. Tryptase release was inhibited by 79–96% at 100 μ M quercetin, kampferol and myricetin, but only 39% by morin; histamine release was inhibited 52–77% by the first three flavonols, but only 28% by morin. These flavonols suppressed intracellular calcium ion elevations in a dose–response manner, with morin being the weakest; they also inhibited phosphorylation of the calcium‐insensitive protein kinase C theta (PKC θ ). 5 Flavonol inhibition of IgE‐mediated proinflammatory mediator release from hCBMCs may be due to inhibition of intracellular calcium influx and PKC θ signaling. Flavonols may therefore be suitable for the treatment of allergic and inflammatory diseases.British Journal of Pharmacology (2005) 145 , 934–944. doi: 10.1038/sj.bjp.0706246