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Effects of chloride substitution on electromechanical responses in the pulmonary artery of Dahl normotensive and hypertensive rats
Author(s) -
Bieger Detlef,
Duggan Jennifer A,
Tabrizchi Reza
Publication year - 2004
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0705703
Subject(s) - medicine , endocrinology , methacholine , blood pressure , chemistry , vascular smooth muscle , perfusion , lung , smooth muscle , respiratory disease
We have investigated the in vitro interaction between chloride ions and endothelium as revealed by alterations in vascular contractility and smooth muscle cell membrane potential in isolated pulmonary arteries from Dahl salt‐resistant normotensive and salt‐sensitive hypertensive rats. Exposure to nitro‐ L ‐arginine methyl ester ( L ‐NAME) of tissues from normotensive but not hypertensive rats augmented contractions to cirazoline. While chloride removal did not alter cirazoline‐induced contractions, it completely abolished the augmentation by L ‐NAME in normotensive rats. However, in hypertensive rats, removal of chloride ions significantly attenuated contractions elicited by cirazoline, and L ‐NAME effectively reversed this inhibition. Methacholine‐induced endothelium‐dependent relaxations of the same magnitude were evident in both normotensive and hypertensive rats. However, basal cyclic GMP levels were found to be significantly higher (7.8‐fold) in blood vessels of normotensive rats compared to hypertensive rats. The resting membrane potential in pulmonary arteries of hypertensive rats (−52.1±1.04 mV) revealed a significant hyperpolarisation when compared with that of normotensive rats (−46.4±1.58 mV). Cirazoline did not produce a significant depolarisation in blood vessels of either normotensive or hypertensive rats. Perfusion with chloride‐free solution resulted in a modest but significant hyperpolarisation (−8.0 mV) in the blood vessels of hypertensive but not in normotensive rats. We conclude that salt‐dependent hypertension in Dahl rats is accompanied by functional and biochemical changes in low‐pressure blood vessels. These changes can, in part, be attributed to impairment in the basal, but not methacholine‐stimulated, release of nitric oxide, and to altered chloride ion handling.British Journal of Pharmacology (2004) 141 , 1068–1076. doi: 10.1038/sj.bjp.0705703