Changes in rectal temperature and ECoG spectral power of sensorimotor cortex elicited in conscious rabbits by i.c.v. injection of GABA, GABA A and GABA B agonists and antagonists

In order to ascertain whether both GABA A and GABA B , or only GABA B receptors, directly modulate thermoregulation in conscious rabbits, GABA A /GABA B agonist and antagonist agents were injected intracerebroventricularly in conscious rabbits while monitoring changes in rectal temperature (RT), gross motor behaviour (GMB) and electrocorticogram (ECoG) power spectra (ps) from sensorimotor cortices. GABA (48 μ mol), nipecotic acid (50 nmol), THIP (60 nmol), muscimol (18 nmol) and baclofen (8 nmol) induced hypothermia (−ΔRTmax values of 1.70±0.1, 1.4±0.2, 1.0±0.4, 1.1±0.2 and 1.6±0.3°C, respectively), accompanied by inhibition of GMB and ECoG synchronization. THIP increased ps at δ frequency band (1.1−3.3 Hz), while GABA, nipecotic acid, muscimol and baclofen did the same at both δ and θ (4.6−6.5 Hz) frequency bands. ECoG ps changes were concomitant or even preceded hypothermia. Bicuculline (1.8 nmol) induced hyperthermia (ΔRTmax 1.2±0.5°C) and slight excitation of GMB, while CGP35348 (1.2 μ mol) did not affect RT nor GMB. Both compounds did not affect ECoG ps. Bicuculline potentiated muscimol‐induced hypothermia, inhibition of GMB and synchronization of ECoG, while CGP35348 fully antagonized these effects. In conclusion, the present results, while confirming the prevailing role of GABA B , also outline a direct involvement of GABA A receptors in the central mechanisms of thermoregulation. Ascending inhibition towards discrete cortical areas controlling muscular activity and thermogenesis may result from GABA receptor activation in neurones proximal to the ventricles, thus contributing to hypothermia, although hypothermia‐induced reduction of neuronal activity of these cortical areas cannot be ruled out.British Journal of Pharmacology (2004) 141 , 152–162. doi: 10.1038/sj.bjp.0705593