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Endosome sorting of β 2 ‐adrenoceptors is GRK5 independent
Author(s) -
Millman Ellen E,
Rosenfeld Jennifer L,
Vaughan David J,
Nguyen Jacqueline,
Dai WenPing,
AlpizarFoster Estrella,
Clark Richard B,
Knoll Brian J,
Moore Robert H
Publication year - 2004
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/sj.bjp.0705504
Subject(s) - internalization , endocytic cycle , radioligand , receptor , endosome , biology , microbiology and biotechnology , kinase , biochemistry , endocytosis
We have investigated the role of G protein‐coupled receptor kinase 5 (GRK5) in the regulation of endosome sorting of human β 2 ‐adrenoceptors. Expressing GRK5 at a high level significantly increased the extent of internalization of wild‐type β 2 ‐adrenoceptors and of an internalization‐defective mutant receptor, and increased receptor phosphorylation at serines 355 and 356 in the cytoplasmic tail. Overexpressing GRK5 did not alter β 2 ‐adrenoceptor recycling as assessed by immunofluorescence microscopy and radioligand binding assays nor was there any change in receptor downregulation. These data indicate that GRK5 does not regulate the sorting of β 2 ‐adrenoceptors in the endocytic pathway.British Journal of Pharmacology (2004) 141 , 277–284. doi: 10.1038/sj.bjp.0705504